Journal:

Article Title: Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

doi: 10.1016/j.virol.2011.01.006

Figure Lengend Snippet: Patterns of host gene expression in AcMNPV-infected Sf21 cells determined by microarray analysis. Probes for host genes designed based on ESTs sequence data from SPODOBASE, http://bioweb.ensam.inra.fr/spodobase/). The approximate number of genes that were A) up-regulated or B) down-regulated during the time course of infection (6, 12, and 24 hpi) compared with the mock-infected controls; C) Chart comparing the expression trends exhibited by up- and down-regulated genes during the time course of infection. Only genes that their level of expression were equal to or more than 1.2 fold change and showed significant difference (p<0.05, ONE WAY ANOVA) were selected.

Article Snippet: Microarray Design A 44K Agilent oligonucleotide microarray chip was designed to include most of the known ESTs of Spodoptera frugiperda .

Techniques: Expressing, Infection, Microarray, Sequencing

Journal:

Article Title: Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

doi: 10.1016/j.virol.2011.01.006

Figure Lengend Snippet: Analysis of non-clathrin coat protein zeta 1-COP gene expression. Non-clathrin coat protein zeta 1-COP gene is represented by two different ESTs in SPODOBASE. A) SF9L03957-Contig1 (EST1) and Sf1P09405-5-1-Contig1 (EST2) that exhibit divergent expression patterns on microarrays. The fold change in expression for each probe, designated p1 - p6, at each time point is the average from four independent biological replicates. B) Diagram shows the relationship between EST1 and 2 and the locations of the microarray probes. Sequence comparisons reveal that both contigs share two common regions of nearly identical nucleotide sequence, indicated by the shaded black bars. The remaining sequences are unique to each EST, and depicted by either unfilled white or shaded grey bars. Probe 1 (p1) matches both contig sequences, p2-p3 are specific for the unique region of EST1, and p4 and p6 are specific for the unique region of EST2. Most of p5 is specific for the unique region of EST2, however it overlaps the small region of sequence identity shared with EST 1 (13 identical nucleotides).

Article Snippet: Microarray Design A 44K Agilent oligonucleotide microarray chip was designed to include most of the known ESTs of Spodoptera frugiperda .

Techniques: Expressing, Microarray, Sequencing

Journal:

Article Title: Comprehensive analysis of host gene expression in Autographa californica nucleopolyhedrovirus-infected Spodoptera frugiperda cells

doi: 10.1016/j.virol.2011.01.006

Figure Lengend Snippet: comparison between the average fold change of microarray and qRT-PCR

Article Snippet: Microarray Design A 44K Agilent oligonucleotide microarray chip was designed to include most of the known ESTs of Spodoptera frugiperda .

Techniques: Microarray

Journal: BMC Biotechnology

Article Title: Low density DNA microarray for detection of most frequent TP53 missense point mutations

doi: 10.1186/1472-6750-5-8

Figure Lengend Snippet: Detection of TP53 silent and missense base substitutions using DNA sequencing versus DNA microarray hybridization.

Article Snippet: In the present work we designed a novel small format DNA chip (genosensor array) to search for nine point mutations in codons 248, 249 and 273 of the TP53 gene.

Techniques: DNA Sequencing, Microarray, Hybridization, Sequencing, Mutagenesis

Journal: BMC Biotechnology

Article Title: Low density DNA microarray for detection of most frequent TP53 missense point mutations

doi: 10.1186/1472-6750-5-8

Figure Lengend Snippet: Layout of the TP53 low density DNA microarray . (A): Names and alignment of stacking oligonucleotides and probes to their respective synthetic wild type target sequence. Bold letters correspond to the nucleotide change in DNA sequence due to the point mutations. (B): Layout of the probe array on the glass slide. The probes were applied to the slide in triplicate as depicted at the top. The numbers correspond to the probes in Table 1.

Article Snippet: In the present work we designed a novel small format DNA chip (genosensor array) to search for nine point mutations in codons 248, 249 and 273 of the TP53 gene.

Techniques: Microarray, Sequencing